PCR ORNi-PCR

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Get tips on using REDExtract-N-Amp™ PCR ReadyMix™ to perform PCR Mouse

Products Sigma-Aldrich REDExtract-N-Amp™ PCR ReadyMix™

I have been doing multiplex PCR and qPCR separately. Is there a way to combine them in a single reaction using Sybr Green?

Discussions Multiplex PCR and qPCR

Get tips on using NucleoSpin® Gel and PCR Clean-up to perform DNA gel extraction / PCR product purification Product size > 15Kb

Products Macherey Nagel NucleoSpin® Gel and PCR Clean-up

Get tips on using EpiTect Methyl II PCR Assays to perform DNA methylation profiling Gene specific profiling - MCF-7 FOXA2

Products Qiagen EpiTect Methyl II PCR Assays

Get tips on using SMARTer® PCR cDNA Synthesis Kit to perform cDNA synthesis Yeast

Products Takara Bio Inc SMARTer® PCR cDNA Synthesis Kit

Get tips on using High Pure PCR Template Preparation Kit to perform DNA isolation / purification Tissue - placenta

Products Roche Lifesciences High Pure PCR Template Preparation Kit

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. The resulting amplicons are generally detected by gel electrophoresis and for some further applications like cloning, sequencing, amplicon product needs to be recovered from the gel and subsequently purified. However, non-specific product amplification and primer-dimer formation during set-up make gel extraction difficult. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

DNA DNA gel extraction / PCR product purification Product size < 15Kb

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. The resulting amplicons are generally detected by gel electrophoresis and for some further applications like cloning, sequencing, amplicon product needs to be recovered from the gel and subsequently purified. However, non-specific product amplification and primer-dimer formation during set-up make gel extraction difficult. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

DNA DNA gel extraction / PCR product purification Product size > 15Kb

Get tips on using EZ-10 Spin Column PCR Product Purification kit to perform DNA gel extraction / PCR product purification Product size > 15Kb

Products Bio Basic EZ-10 Spin Column PCR Product Purification kit

Get tips on using High Pure PCR Template Preparation Kit to perform DNA isolation / purification Cells - Primary cells Rat astrocytes

Products Roche Lifesciences High Pure PCR Template Preparation Kit

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